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kb dna substrate  (New England Biolabs)


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    Structured Review

    New England Biolabs kb dna substrate
    Kb Dna Substrate, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1840 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lambda+dna/Lambda+DNA/pm42913629-185-2-8
    Average 97 stars, based on 1840 article reviews
    kb dna substrate - by Bioz Stars, 2026-11
    97/100 stars

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    Related Articles

    Lambda DNA Preparation:

    Article Title: Biocatalytic synthesis of S-adenosyl-l-methionine analogues for allosteric activation of cystathionine-β-synthase.
    Article Snippet: Methylation of lambda DNA by SAM analogues was performed using a commercial kit (M0211S, New England Biolabs).

    Article Title: Detection of known gene fusions in cancer cell lines using whole-genome bisulfite sequencing data.
    Article Snippet: Spike-in controls AR TIC LE IN PR ES S consisting of pUC19 (0.1%) and lambda DNA (0.1%) from the NEBNext Enzymatic Methyl-seq Kit (New England Biolabs) were added to the sheared DNA.

    Article Title: Biocatalytic synthesis of S-adenosyl-l-methionine analogues for allosteric activation of cystathionine-β-synthase.
    Article Snippet: Reactions of 25 μL containing 0.5 μg Lambda DNA (N3013S, New England Biolabs), 20U EcoRI methyltransferase (M0211S, New England Biolabs), 10× Methyltransferase buffer (M0211S, New England Biolabs) and 10 μM commercial SAM (B9003S, New England Biolabs) or SAM analogue (10 μM, as per phosphate) were incubated at 37 °C for 1 h, after which EcoRI methyltransferase was inactivated by heating to 65 °C for 20min.

    Article Title: Recognition and remodelling of nucleosomes and hexasomes by the human INO80 complex
    Article Snippet: Reactions were stopped by adding Lambda DNA (0.2 mg/ml; NEB).

    Article Title: RPA directly stimulates Mer3 helicase processivity to ensure normal crossover formation in meiosis
    Article Snippet: The dsDNA hairpin was obtained by PCR amplification with Phusion High-Fidelity DNA Polymerase (Thermo Scientific) using Lambda DNA (New England Biolabs) as template and oligonucleotides that include different BsaI restriction sites in each side of the PCR fragment (see Supplementary Tables and for oligonucleotides) followed by purification (QIAGEN) as described previously .

    Article Title: Detection of known gene fusions in cancer cell lines using whole-genome bisulfite sequencing data
    Article Snippet: Spike-in controls consisting of pUC19 (0.1%) and lambda DNA (0.1%) from the NEBNext Enzymatic Methyl-seq Kit (New England Biolabs) were added to the sheared DNA.

    Article Title: 3 RAD ‐Guided SNP Discovery for Species Identification and Conservation of the Medicinal Southern African Tree Genus Greyia Hook. & Harv.
    Article Snippet: The integrity and quantity of DNA extracted from the ascertainment panel plants was first evaluated on a 1% agarose gel, alongside a dilution series of lambda DNA (New England Biolabs, Massachusetts) ranging from 8 ng to 67 ng.

    Article Title: In‑Situ ssDNA Isolation from dsDNA Sources as a Streamlined Pathway to DNA Origami Assembly and Testing
    Article Snippet: Lambda DNA, pUC19, and phiX174 single-stranded and supercoiled DNA were purchased from New England Biolabs (N3011S, N3041S, N3021S, N3023S).

    Incubation:

    Article Title: Biocatalytic synthesis of S-adenosyl-l-methionine analogues for allosteric activation of cystathionine-β-synthase.
    Article Snippet: Methylation of lambda DNA by SAM analogues was performed using a commercial kit (M0211S, New England Biolabs).

    Article Title: Detection of known gene fusions in cancer cell lines using whole-genome bisulfite sequencing data.
    Article Snippet: Spike-in controls AR TIC LE IN PR ES S consisting of pUC19 (0.1%) and lambda DNA (0.1%) from the NEBNext Enzymatic Methyl-seq Kit (New England Biolabs) were added to the sheared DNA.

    Article Title: Biocatalytic synthesis of S-adenosyl-l-methionine analogues for allosteric activation of cystathionine-β-synthase.
    Article Snippet: Reactions of 25 μL containing 0.5 μg Lambda DNA (N3013S, New England Biolabs), 20U EcoRI methyltransferase (M0211S, New England Biolabs), 10× Methyltransferase buffer (M0211S, New England Biolabs) and 10 μM commercial SAM (B9003S, New England Biolabs) or SAM analogue (10 μM, as per phosphate) were incubated at 37 °C for 1 h, after which EcoRI methyltransferase was inactivated by heating to 65 °C for 20min.

    Article Title: Recognition and remodelling of nucleosomes and hexasomes by the human INO80 complex
    Article Snippet: Reactions were stopped by adding Lambda DNA (0.2 mg/ml; NEB).

    Article Title: RPA directly stimulates Mer3 helicase processivity to ensure normal crossover formation in meiosis
    Article Snippet: The dsDNA hairpin was obtained by PCR amplification with Phusion High-Fidelity DNA Polymerase (Thermo Scientific) using Lambda DNA (New England Biolabs) as template and oligonucleotides that include different BsaI restriction sites in each side of the PCR fragment (see Supplementary Tables and for oligonucleotides) followed by purification (QIAGEN) as described previously .

    Article Title: Detection of known gene fusions in cancer cell lines using whole-genome bisulfite sequencing data
    Article Snippet: Spike-in controls consisting of pUC19 (0.1%) and lambda DNA (0.1%) from the NEBNext Enzymatic Methyl-seq Kit (New England Biolabs) were added to the sheared DNA.

    Article Title: 3 RAD ‐Guided SNP Discovery for Species Identification and Conservation of the Medicinal Southern African Tree Genus Greyia Hook. & Harv.
    Article Snippet: The integrity and quantity of DNA extracted from the ascertainment panel plants was first evaluated on a 1% agarose gel, alongside a dilution series of lambda DNA (New England Biolabs, Massachusetts) ranging from 8 ng to 67 ng.

    Article Title: In‑Situ ssDNA Isolation from dsDNA Sources as a Streamlined Pathway to DNA Origami Assembly and Testing
    Article Snippet: Lambda DNA, pUC19, and phiX174 single-stranded and supercoiled DNA were purchased from New England Biolabs (N3011S, N3041S, N3021S, N3023S).

    Agarose Gel Electrophoresis:

    Article Title: Biocatalytic synthesis of S-adenosyl-l-methionine analogues for allosteric activation of cystathionine-β-synthase.
    Article Snippet: Methylation of lambda DNA by SAM analogues was performed using a commercial kit (M0211S, New England Biolabs).

    Article Title: Detection of known gene fusions in cancer cell lines using whole-genome bisulfite sequencing data.
    Article Snippet: Spike-in controls AR TIC LE IN PR ES S consisting of pUC19 (0.1%) and lambda DNA (0.1%) from the NEBNext Enzymatic Methyl-seq Kit (New England Biolabs) were added to the sheared DNA.

    Article Title: Biocatalytic synthesis of S-adenosyl-l-methionine analogues for allosteric activation of cystathionine-β-synthase.
    Article Snippet: Reactions of 25 μL containing 0.5 μg Lambda DNA (N3013S, New England Biolabs), 20U EcoRI methyltransferase (M0211S, New England Biolabs), 10× Methyltransferase buffer (M0211S, New England Biolabs) and 10 μM commercial SAM (B9003S, New England Biolabs) or SAM analogue (10 μM, as per phosphate) were incubated at 37 °C for 1 h, after which EcoRI methyltransferase was inactivated by heating to 65 °C for 20min.

    Article Title: Recognition and remodelling of nucleosomes and hexasomes by the human INO80 complex
    Article Snippet: Reactions were stopped by adding Lambda DNA (0.2 mg/ml; NEB).

    Article Title: RPA directly stimulates Mer3 helicase processivity to ensure normal crossover formation in meiosis
    Article Snippet: The dsDNA hairpin was obtained by PCR amplification with Phusion High-Fidelity DNA Polymerase (Thermo Scientific) using Lambda DNA (New England Biolabs) as template and oligonucleotides that include different BsaI restriction sites in each side of the PCR fragment (see Supplementary Tables and for oligonucleotides) followed by purification (QIAGEN) as described previously .

    Article Title: Detection of known gene fusions in cancer cell lines using whole-genome bisulfite sequencing data
    Article Snippet: Spike-in controls consisting of pUC19 (0.1%) and lambda DNA (0.1%) from the NEBNext Enzymatic Methyl-seq Kit (New England Biolabs) were added to the sheared DNA.

    Article Title: 3 RAD ‐Guided SNP Discovery for Species Identification and Conservation of the Medicinal Southern African Tree Genus Greyia Hook. & Harv.
    Article Snippet: The integrity and quantity of DNA extracted from the ascertainment panel plants was first evaluated on a 1% agarose gel, alongside a dilution series of lambda DNA (New England Biolabs, Massachusetts) ranging from 8 ng to 67 ng.

    Article Title: In‑Situ ssDNA Isolation from dsDNA Sources as a Streamlined Pathway to DNA Origami Assembly and Testing
    Article Snippet: Lambda DNA, pUC19, and phiX174 single-stranded and supercoiled DNA were purchased from New England Biolabs (N3011S, N3041S, N3021S, N3023S).

    Polymerase Chain Reaction:

    Article Title: Biocatalytic synthesis of S-adenosyl-l-methionine analogues for allosteric activation of cystathionine-β-synthase.
    Article Snippet: Methylation of lambda DNA by SAM analogues was performed using a commercial kit (M0211S, New England Biolabs).

    Article Title: Detection of known gene fusions in cancer cell lines using whole-genome bisulfite sequencing data.
    Article Snippet: Spike-in controls AR TIC LE IN PR ES S consisting of pUC19 (0.1%) and lambda DNA (0.1%) from the NEBNext Enzymatic Methyl-seq Kit (New England Biolabs) were added to the sheared DNA.

    Article Title: Biocatalytic synthesis of S-adenosyl-l-methionine analogues for allosteric activation of cystathionine-β-synthase.
    Article Snippet: Reactions of 25 μL containing 0.5 μg Lambda DNA (N3013S, New England Biolabs), 20U EcoRI methyltransferase (M0211S, New England Biolabs), 10× Methyltransferase buffer (M0211S, New England Biolabs) and 10 μM commercial SAM (B9003S, New England Biolabs) or SAM analogue (10 μM, as per phosphate) were incubated at 37 °C for 1 h, after which EcoRI methyltransferase was inactivated by heating to 65 °C for 20min.

    Article Title: Recognition and remodelling of nucleosomes and hexasomes by the human INO80 complex
    Article Snippet: Reactions were stopped by adding Lambda DNA (0.2 mg/ml; NEB).

    Article Title: RPA directly stimulates Mer3 helicase processivity to ensure normal crossover formation in meiosis
    Article Snippet: The dsDNA hairpin was obtained by PCR amplification with Phusion High-Fidelity DNA Polymerase (Thermo Scientific) using Lambda DNA (New England Biolabs) as template and oligonucleotides that include different BsaI restriction sites in each side of the PCR fragment (see Supplementary Tables and for oligonucleotides) followed by purification (QIAGEN) as described previously .

    Article Title: Detection of known gene fusions in cancer cell lines using whole-genome bisulfite sequencing data
    Article Snippet: Spike-in controls consisting of pUC19 (0.1%) and lambda DNA (0.1%) from the NEBNext Enzymatic Methyl-seq Kit (New England Biolabs) were added to the sheared DNA.

    Article Title: 3 RAD ‐Guided SNP Discovery for Species Identification and Conservation of the Medicinal Southern African Tree Genus Greyia Hook. & Harv.
    Article Snippet: The integrity and quantity of DNA extracted from the ascertainment panel plants was first evaluated on a 1% agarose gel, alongside a dilution series of lambda DNA (New England Biolabs, Massachusetts) ranging from 8 ng to 67 ng.

    Article Title: In‑Situ ssDNA Isolation from dsDNA Sources as a Streamlined Pathway to DNA Origami Assembly and Testing
    Article Snippet: Lambda DNA, pUC19, and phiX174 single-stranded and supercoiled DNA were purchased from New England Biolabs (N3011S, N3041S, N3021S, N3023S).

    Amplification:

    Article Title: Biocatalytic synthesis of S-adenosyl-l-methionine analogues for allosteric activation of cystathionine-β-synthase.
    Article Snippet: Methylation of lambda DNA by SAM analogues was performed using a commercial kit (M0211S, New England Biolabs).

    Article Title: Detection of known gene fusions in cancer cell lines using whole-genome bisulfite sequencing data.
    Article Snippet: Spike-in controls AR TIC LE IN PR ES S consisting of pUC19 (0.1%) and lambda DNA (0.1%) from the NEBNext Enzymatic Methyl-seq Kit (New England Biolabs) were added to the sheared DNA.

    Article Title: Biocatalytic synthesis of S-adenosyl-l-methionine analogues for allosteric activation of cystathionine-β-synthase.
    Article Snippet: Reactions of 25 μL containing 0.5 μg Lambda DNA (N3013S, New England Biolabs), 20U EcoRI methyltransferase (M0211S, New England Biolabs), 10× Methyltransferase buffer (M0211S, New England Biolabs) and 10 μM commercial SAM (B9003S, New England Biolabs) or SAM analogue (10 μM, as per phosphate) were incubated at 37 °C for 1 h, after which EcoRI methyltransferase was inactivated by heating to 65 °C for 20min.

    Article Title: Recognition and remodelling of nucleosomes and hexasomes by the human INO80 complex
    Article Snippet: Reactions were stopped by adding Lambda DNA (0.2 mg/ml; NEB).

    Article Title: RPA directly stimulates Mer3 helicase processivity to ensure normal crossover formation in meiosis
    Article Snippet: The dsDNA hairpin was obtained by PCR amplification with Phusion High-Fidelity DNA Polymerase (Thermo Scientific) using Lambda DNA (New England Biolabs) as template and oligonucleotides that include different BsaI restriction sites in each side of the PCR fragment (see Supplementary Tables and for oligonucleotides) followed by purification (QIAGEN) as described previously .

    Article Title: Detection of known gene fusions in cancer cell lines using whole-genome bisulfite sequencing data
    Article Snippet: Spike-in controls consisting of pUC19 (0.1%) and lambda DNA (0.1%) from the NEBNext Enzymatic Methyl-seq Kit (New England Biolabs) were added to the sheared DNA.

    Article Title: 3 RAD ‐Guided SNP Discovery for Species Identification and Conservation of the Medicinal Southern African Tree Genus Greyia Hook. & Harv.
    Article Snippet: The integrity and quantity of DNA extracted from the ascertainment panel plants was first evaluated on a 1% agarose gel, alongside a dilution series of lambda DNA (New England Biolabs, Massachusetts) ranging from 8 ng to 67 ng.

    Article Title: In‑Situ ssDNA Isolation from dsDNA Sources as a Streamlined Pathway to DNA Origami Assembly and Testing
    Article Snippet: Lambda DNA, pUC19, and phiX174 single-stranded and supercoiled DNA were purchased from New England Biolabs (N3011S, N3041S, N3021S, N3023S).

    Purification:

    Article Title: Biocatalytic synthesis of S-adenosyl-l-methionine analogues for allosteric activation of cystathionine-β-synthase.
    Article Snippet: Methylation of lambda DNA by SAM analogues was performed using a commercial kit (M0211S, New England Biolabs).

    Article Title: Detection of known gene fusions in cancer cell lines using whole-genome bisulfite sequencing data.
    Article Snippet: Spike-in controls AR TIC LE IN PR ES S consisting of pUC19 (0.1%) and lambda DNA (0.1%) from the NEBNext Enzymatic Methyl-seq Kit (New England Biolabs) were added to the sheared DNA.

    Article Title: Biocatalytic synthesis of S-adenosyl-l-methionine analogues for allosteric activation of cystathionine-β-synthase.
    Article Snippet: Reactions of 25 μL containing 0.5 μg Lambda DNA (N3013S, New England Biolabs), 20U EcoRI methyltransferase (M0211S, New England Biolabs), 10× Methyltransferase buffer (M0211S, New England Biolabs) and 10 μM commercial SAM (B9003S, New England Biolabs) or SAM analogue (10 μM, as per phosphate) were incubated at 37 °C for 1 h, after which EcoRI methyltransferase was inactivated by heating to 65 °C for 20min.

    Article Title: Recognition and remodelling of nucleosomes and hexasomes by the human INO80 complex
    Article Snippet: Reactions were stopped by adding Lambda DNA (0.2 mg/ml; NEB).

    Article Title: RPA directly stimulates Mer3 helicase processivity to ensure normal crossover formation in meiosis
    Article Snippet: The dsDNA hairpin was obtained by PCR amplification with Phusion High-Fidelity DNA Polymerase (Thermo Scientific) using Lambda DNA (New England Biolabs) as template and oligonucleotides that include different BsaI restriction sites in each side of the PCR fragment (see Supplementary Tables and for oligonucleotides) followed by purification (QIAGEN) as described previously .

    Article Title: Detection of known gene fusions in cancer cell lines using whole-genome bisulfite sequencing data
    Article Snippet: Spike-in controls consisting of pUC19 (0.1%) and lambda DNA (0.1%) from the NEBNext Enzymatic Methyl-seq Kit (New England Biolabs) were added to the sheared DNA.

    Article Title: 3 RAD ‐Guided SNP Discovery for Species Identification and Conservation of the Medicinal Southern African Tree Genus Greyia Hook. & Harv.
    Article Snippet: The integrity and quantity of DNA extracted from the ascertainment panel plants was first evaluated on a 1% agarose gel, alongside a dilution series of lambda DNA (New England Biolabs, Massachusetts) ranging from 8 ng to 67 ng.

    Article Title: In‑Situ ssDNA Isolation from dsDNA Sources as a Streamlined Pathway to DNA Origami Assembly and Testing
    Article Snippet: Lambda DNA, pUC19, and phiX174 single-stranded and supercoiled DNA were purchased from New England Biolabs (N3011S, N3041S, N3021S, N3023S).



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